First Molecular Identification of Tetranychus malaysiensis and Sancassania sp. in Bangladesh

Abstract

The mite samples were collected from different host plants in southern part of Bangladesh during January, 2017 to December, 2017. Morphological identification was performed in Systematic Entomology laboratory of Patuakhali Science and Technology University, Bangladesh. Consequently, molecular work was carried out in the Insect Molecular Physiology Laboratory of Kyungpook National University, Korea using both of ribosomal internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome oxidase subunit I (mtCOI) primers. Molecularly three species (Tetranychus truncatus, Tetranychus malaysiensis and Sancassania sp.) were identified by those nucleotide sequences which make different clades in phylogenetic tree with distinct distance. Analysis of pairwise distance of nucleotides and different clades in a phylogeny were shown great host range diversity of red acaroid mite among 9 samples from different host plants. The red acaroid mite collected from sesame, sunflower, mungbean, okra and jute which were morphologically little bit different but molecularly identical, which were clustered in same clade of phylogeny. Sancassania sp. were shown another clade in phylogenetic tree which were collected from aroid and cucurbits. In this study, using mtCOI sequences of Tetranychus malaysiensis and ITS2 sequences of Sancassania sp. provide molecular identification for first time in Bangladesh