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Effect of azadirachtin on digestive enzyme activity and gene expression of sweetpotato whitefly, Bemisia tabaci

Md. Asaduzzaman, Kyeong-Yeoll Lee

Abstract


Pure compound of azadirachtin tested against adult B. tabaci using artificial diet. Digestive enzymes activity was observed after feeding with 5 ppm azadirachtin for 12 hours. The specific activity of the tested 3 protease enzymes- trypsin, chymotrypsin and aminopeptidase were down regulated. The transcript level of eight genes, two stresses, and six cytoskeleton genes were observed feeding with azadirachtin 5 ppm for 12 hours. Among the tested genes, the expression level of all genes, hsp70, hsp90, p8 protein, myosin, profiling, myofilin, paramryosin, tropomyosin were down regulated.

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